发明名称 |
SYSTEMS AND METHODS FOR IDENTIFYING PROTEIN STABILIZERS |
摘要 |
A device for studying protein conformation transformation can include a macroscopic substrate, and chaperonin proteins bound to the substrate, each chaperonin protein being capable of binding to a protein of interest during or after undergoing protein conformation transformation. The device may also include the proteins of interest bound to the substrate, where the substrate is included in a label-free assay system. A method of studying protein conformation transformation can include: providing a macroscopic substrate bound with the chaperonin protein and immersing the chaperonin protein in a study composition having the protein of interest, or include providing a macroscopic substrate bound with the protein of interest; and immersing the protein in a study composition having the chaperonin. Such a method can be done with and without a potential stabilizer in order to determine whether the potential stabilizer stabilizes the protein of interest. |
申请公布号 |
US2015094216(A1) |
申请公布日期 |
2015.04.02 |
申请号 |
US201314381375 |
申请日期 |
2013.02.26 |
申请人 |
Fisher Mark T.;Naik Subhashchandra |
发明人 |
Fisher Mark T.;Naik Subhashchandra |
分类号 |
G01N33/68;G01N33/543 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
1. A device for studying protein conformation transformation, the device comprising:
a macroscopic substrate; and one or more chaperonin proteins or functional portion thereof bound to the substrate, each chaperonin protein being capable of binding to a protein of interest during or after undergoing protein conformation transformation, the protein of interest being capable of undergoing protein conformation transformation at physiological conditions. |
地址 |
Prairie Villiage KS US |