ISFET ARRAY FOR DETECTING A SINGLE NUCLEOTIDE POLYMORPHISM

摘要

Apparatus comprising a module for detecting a single nucleotide polymorphism, SNP, within a genome and comprising: four reaction chambers for receiving a fluid sample, the reaction chambers each containing a different one of the bases A, C, G, T, and a primer; a reference electrode which in use is immersed in said fluid sample; a pair of Ion Sensitive Field Effect Transistors, ISFETs, associated with each reaction chamber, the ISFETs of each pair comprising respective sensing membranes, or a common sensing membrane, exposed within the associated reaction chamber; and a plurality of difference detectors, each difference detector having a pair of inputs coupled respectively to outputs of ISFETs associated with different reaction chambers.

主权项

1. Apparatus comprising a module for detecting a single nucleotide polymorphism, SNP, within a genome and comprising:
four reaction chambers for receiving a fluid sample, the reaction chambers each containing a different, single one of the bases A, C, G, T, and an identical primer; a reference electrode which in use is immersed in said fluid sample; a pair of Ion Sensitive Field Effect Transistors, ISFETs, within each reaction chamber, the ISFETs of each pair comprising respective sensing membranes, or a common sensing membrane, exposed within the associated reaction chamber; and a plurality of difference detectors, each difference detector having a pair of inputs coupled respectively to outputs of ISFETs associated with different reaction chambers.