摘要 |
PROBLEM TO BE SOLVED: To provide an aromatic-substituted xanthene compound that is useful as a fluorescent dye for use in the independent detection of multiple spatially overlapping analytes in a mixture, e.g, single-tube multiplex DNA probe assays, immuno assays, multicolor DNA sequencing methods, and the like.SOLUTION: The present invention provides a composition of the formula (Yand Yseparately selected from the group consisting of hydroxyl, oxygen, imminium, linking group and amine, or Ytogether with Ris cyclic amine, or Ytogether with Ris cyclic imine; R, R, R, and Rseparately selected from the group consisting of hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, imminium, amido, nitrile, lower alkoxy, phenyl, and linking group). |