APPLICATION OF ENZYME-QUANTUM DOTS HYBRID SYSTEM FOR THE DETERMINATION OF URIC ACID

摘要

The present invention relates to a uricase/HRP-CdS QDs hydrid system for the determination of uric acid. The QDs was used as an electron donor, whereas uricase and HRP (horseradish peroxidase) were used as acceptors for the oxidation/reduction reactions involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence which was proportional to uric acid concentration. The hybrid system demonstrated maximum activity of uricase/HRP at ratio of 1/1 and pH 7.0, respectively. The linearity of the hybrid system towards uric acid was in the concentration range of 125-1000μΜwith detection limit of 125μΜ.