发明名称 |
Preparation of Cryogenic Sample for Charged-Particle Microscopy |
摘要 |
A method of preparing a sample for a charged-particle microscope includes:
Providing a substantially plate-like sample holder having opposed first and second major surfaces substantially parallel to one another, comprising at least one aperture connecting said major surfaces and across which a membrane has been spanned upon said first major surface, which membrane comprises at least one perforation;Spanning a film of liquid across said perforation, which liquid comprises at least one study specimen suspended therein;Plunging the sample holder onto a bath of cryogen, whereby the sample holder is held with said first major surface pointing toward the cryogen and arranged substantially parallel to an exposed surface of the cryogen; andApplying a blast of cryogenic fluid to said film from a nozzle pointing toward said second major surface, immediately prior to the film making contact with said cryogen.;A corresponding apparatus is also described. |
申请公布号 |
US2015090878(A1) |
申请公布日期 |
2015.04.02 |
申请号 |
US201414498712 |
申请日期 |
2014.09.26 |
申请人 |
FEI Company |
发明人 |
Rémigy Hervé-William;Smulders-Weemers Karin;Ovsyanko Mikhail Mikhaylovich;Nijpels Frank;Sader Kasim Stefan |
分类号 |
G01N1/42;H01J37/20;H01J37/26 |
主分类号 |
G01N1/42 |
代理机构 |
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代理人 |
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主权项 |
1. A method of preparing a sample for study in a charged-particle microscope, comprising the following steps:
providing a substantially plate-like sample holder having opposed first and second major surfaces substantially parallel to one another, comprising at least one aperture that connects said major surfaces and across which a membrane has been spanned upon said first major surface, which membrane comprises at least one perforation; spanning a film of aqueous liquid across said perforation, which liquid comprises at least one study specimen suspended therein; plunging the sample holder onto a bath of cryogen, whereby the sample holder is held with said first major surface pointing toward the cryogen and arranged substantially parallel to an exposed surface of the cryogen; and applying a blast of cryogenic fluid to said film from a nozzle pointing toward said second major surface, immediately prior to the film making contact with said cryogen. |
地址 |
Hillsboro OR US |