Method of MS Mass Spectrometry

摘要

A method of mass spectrometry is disclosed comprising alternating between a first mode in which parent ions are analysed and a second mode in which parent ions are fragmented and their fragment ions are mass analysed. In the first mode the parent ions are charge reduced before being analysed, so as to simplify the parent ion spectral data obtained. In the second mode, the parent ions are not charge reduced prior to fragmentation, so that it remains relatively easy to induce the parent ions to fragment. The parent ions are then associated with their fragment ions using the mass spectral data obtained.

主权项

1. A method of mass spectrometry comprising:
(i) providing a plurality of parent ions; (ii) reducing a charge state of parent ions by subjecting the parent ions to charge reduction conditions, and mass analysing the resulting charge reduced parent ions so as to obtain first mass spectral data; (iii) fragmenting parent ions to produce fragment ions without having first reduced the charge state of the parent ions by exposing the ions to said charge reduction conditions, and mass analysing said fragment ions to obtain second mass spectral data; (iv) wherein the parent ions are alternately and repeatedly subjected to said charge reduction conditions and said fragmenting so that the method repeatedly alternates between steps (ii) and (iii); and wherein the method comprises associating parent ions detected in said first mass spectral data with fragment ions detected in said second mass spectral data.