ENZYME COMPOSITION FOR DNA END REPAIR, ADENYLATION, PHOSPHORYLATION

摘要

Enzyme compositions and their method of use that provide ready-to-use master mixtures. Thexcompositions comprise a modified thermophilic DNA polymerase lacking 5'-3' and 3'-5' exonucleasexactivity premixed with T4 DNA polymerase, Klenow fragment and T4 polynucleotide kinase and all otherxnecessary components, including reaction buffer and nucleoside triphosphates, required to perform DNAxblunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps. Among other benefits, the mixture of different enzymes, buffers and nucleoside triphosphates is stable during prolonged storage.