| 摘要 |
<p>The invention discloses a method for directly detecting and typing nucleic acids of pathogenic microorganism without amplification and a related kit, the invention achieves detecting and typing nucleic acids of pathogenic microorganism without amplification by the combination of multiprobe and the layer by layer assembly of fluorescence quantum dots. The method according to the invention can directly detect a nucleic acid with low concentration without amplification; the multiprobe prevents the false positives which are likely to occur in the process of signal amplification and thus increases detection accuracy. Such technology can achieve the real-time detection and simultaneous genotyping of pathogenic microorganisms with rapid speed and low cost.</p> |
