Dried and irradiated skin equivalents for ready use

摘要

The present invention relates generally to systems and methods for preparing, storing, shipping and using skin equivalents made by organotypic culture. In particular, the present invention relates to systems and methods for producing, transporting, storing and using skin equivalents produced by organotypic culture at reduced temperatures, preferably from 2-8 degrees Celsius to ambient temperature. The methods include sterile packaging of the grafts so that the sterility and integrity of the package is maintained until the time of use for grafting purposes.

主权项

1. A method for treating a wound in a subject comprising:
applying an isolated, non-viable, in vitro human skin equivalent to said wound in said subject under conditions such that said skin equivalent contacts said wound and said wound is treated, wherein the skin equivalent comprises organotypically cultured keratinocytes that have stratified into squamous epithelia; and cell viability in the skin equivalent has been eliminated.