Multimodal platform for nonlinear optical microscopy and microspectroscopy

摘要

A method for providing images using a multimodal nonlinear optical microscope is disclosed. The method includes providing a foundation femtosecond laser beam, generating a first femtosecond laser beam and a second femtosecond laser beam corresponding to the foundation femtosecond laser beam, combining the first femtosecond laser beam and the foundation femtosecond laser beam to generate a first combination femtosecond laser beam, and generating a coherent anti-Stokes Raman scattering (CARS) signal based on the first combination femtosecond laser beam. A multimodal nonlinear optical microscopy platform is also disclosed.

主权项

1. A method for imaging a sample, the method comprising:
providing a foundation femtosecond laser beam; generating a first femtosecond laser beam and a separate second femtosecond laser beam corresponding to the foundation femtosecond laser beam; combining the first femtosecond laser beam and the foundation femtosecond laser beam to generate a first combination femtosecond laser beam; exposing the sample to the first combination femtosecond laser beam, so that the sample produces an optical signal; generating a coherent anti-Stokes Raman scattering (CARS) signal by filtering the optical signal using a first filter; and generating a two-photon fluorescence (TPF) signal by filtering the optical signal using a second, different filter.