Vectors and methods for improved plant transformation efficiency

摘要

Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

主权项

1. A method for transforming plant cells comprising:
contacting a plurality of plant cells with bacterial cells competent for the transformation of said plant cells, wherein the bacterial cells comprise a DNA construct comprising:
i) at least one T-DNA border region;ii) at least one heterologous transgene adjacent to the border region;iii) a coding region for a bacterial selectable marker; andiv) at least one segment of DNA, comprising a cis and/or trans element of a repABC replication origin; and selecting a transformed plant cell based on the presence of at least one heterologous transgene from the DNA construct and the absence of vector backbone DNA from the DNA construct; wherein the at least one segment of DNA comprises a sequence having at least 90% identity to a sequence selected from the group consisting of: SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, and SEQ ID NO: 36.