| 摘要 |
In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an amino acid or peptide linker. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an amino acid or peptide linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention. |
| 主权项 |
1. A fluorescent dye having the formula:
{R1-(L1)a-(AA)n}y-(L2)b-X wherein R1 is a fluorescent dye moiety; AA is an anionic amino acid; n is a selected from the integers 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, and when n is two or greater, each n amino acid is independently selected; X is a member selected from a polyvalent moiety, and a moiety having the structure:wherein
Y is a nucleobase; and u is selected from the integers 1, 2, 3, 4, 5, 6, 7 and 8;y is selected from the integers 1, 2, 3, 4, 5, 6, 7 and 8, such that when y is 2 or greater, X is a polyvalent moiety;
L1 and L2 are selected from bonds and adaptors; a is selected from the integers 0 and 1; and b is an integer selected from 0, 1, 2, 3, 4, 5, 6, 7 and 8. |
