Vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production

摘要

The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimized nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.

主权项

1. A host cell containing a nucleic acid molecule, which is operably linked to a heterologous promoter, and which encodes a polypeptide comprising SEQ ID NO: 7 or an amino acid sequence that is at least 90% identical to SEQ ID NO: 7; wherein the polypeptide has an in vitro and/or in vivo pentose isomerase function; and wherein the host cell is a Saccharomyces cerevisiae cell.