Method of testing for a milk trisaccharide

摘要

A method of testing for a trisaccharide in milk includes the step of providing a predetermined amount of milk. The method also includes the step of isolating a trisachharide of the milk with a hydride insertion reaction to identify the molecule as phosphorylated. Also included in the method is the step of obtaining a mass spectrum of the isolated milk trisaccharide sample. The method further includes the step of identifying a molecular structure of the isolated milk trisaccharide sample.

主权项

1. A method of testing for a trisaccharide in milk comprising the steps of:
providing a predetermined amount of milk; combining the predetermined amount of milk with ethanol, wherein the ethanol and milk are combined in a 10:1 ethanol to milk ratio; isolating a trisaccharide from the milk and ethanol combination to obtain a sample; obtaining a mass spectrum of the isolated milk trisaccharide sample; and identifying a molecular structure of the isolated milk trisaccharide sample, wherein the identified structure is 3′-N-acetyl neuraminyll lactose 6′-phosphate.