SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS MREJ TYPE VII STRAINS

摘要

The present invention describes novel SCCmec right extremity junction (MREJ) sequences for the detection of methicillin-resistant Staphyloccocus aureus (MRSA). The MRSA strain includes an SCCmec element containing a mecA gene inserted into chromosomal DNA, thereby generating a polymorphic MREJ type vii sequence that comprises polymorphic sequences from the SCCmec element right extremity and chromosomal DNA adjoining the extrimity. The present invention concerns the use of MREJ type vii sequences for diagnostic purposes. Disclosed, is a method for detecting the presence of at least one MREJ type vii MRSA strain comprising: a) contacting a sample with a first primer and a second primer to specifically generate an amplicon; and b) detecting the presence of the amplicon. The primers hybridize with polymorphic sequences from the SCCmec element right extremity of an MREJ type vii and with a chromosomal sequence of S. aureus.