Integrated analytical system and method

摘要

An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.

主权项

1. An analytical device comprising a chip comprising:
an array of zero mode waveguides in a transparent material, each zero mode waveguide having a sample within it, and each zero mode waveguide connected to a fluid conduit for bringing fluid to the zero mode waveguide, the fluid conduit surrounded by an opaque material preventing illumination light from entering the fluid conduit and preventing illumination light from entering a zero mode waveguide from above; wherein the chip is configured such that the illumination light enters the chip from above and is directed up into the bottom of the zero mode waveguides by reflective optical elements below the zero mode waveguides; wherein the illumination light stimulates emission from the sample within the zero mode waveguide into the transparent material to a detector below the zero mode waveguide.