Enzymatic hydrolysis pretreatment of lignocellulosic materials

摘要

This disclosure relates to a method for treating a biomass comprising a lignocellulosic material to produce fermentable sugars comprising the steps of treating the biomass to produce a biomass with a depolymerized lignin, adding a carbonyl scavenger to the biomass before, during, or after said treating step to inhibit repolymerization of the lignin, adding at least one of a laccase enzyme and a cellulases enzyme to the biomass with depolymerized lignin subsequently to the addition of the carbonyl scavenger, and producing a fermentable sugar from the action of the laccase enzyme and cellulases enzyme on the biomass with depolymerized lignin.

主权项

1. A method for treating a biomass comprising a lignocellulosic material to produce fermentable sugars through saccharification comprising:
treating the biomass to produce a biomass with a depolymerized lignin; adding a carbonium-ion scavenger to the biomass before, during, or after said treating step to inhibit repolymerization of the lignin; adding a laccase enzyme and a cellulase enzyme to the biomass with depolymerized lignin subsequently to the addition of the carbonium-ion scavenger; and producing a fermentable sugar from the enzymatic actions of the laccase enzyme and cellulase enzyme in the presence of the carbonium-ion scavenger on the biomass with depolymerized lignin; wherein the carbonium-ion scavenger is 2-naphthol.