| 主权项 |
1. A method for sequencing a target nucleic acid molecule, the method comprising:
(a) providing a first nucleic acid molecule and a second nucleic acid molecule, the first nucleic acid molecule and the second nucleic acid molecule comprising the same sequence as the target nucleic acid molecule, wherein the first nucleic acid molecule is complexed with a first primer and a first polymerase and the second nucleic acid molecule is complexed with a second primer and a second polymerase, wherein the first polymerase has a different rate of polymerization from the second polymerase, and further wherein the first nucleic acid molecule is a first circular template and the second nucleic acid molecule is a second circular template; (b) subjecting the first circular template to a first polymerization reaction to yield a first strand complementary to the first nucleic acid molecule; (c) subjecting the second circular template to a second polymerization reaction to yield a second strand complementary to the second nucleic acid molecule, wherein the first polymerization reaction is asynchronous compared to the second polymerization reaction, the asynchronous due to different rates of polymerization exhibited by the first polymerase on the first circular template and the second polymerase on the second circular template; (d) obtaining overlapping time sequences of incorporation of nucleotides or nucleotide analogs into the first strand and the second strand during the asynchronous polymerization reactions; and (e) sequencing the target nucleic acid molecule by compiling, using a programmed computer, the overlapping time sequences of incorporation of nucleotides into the first and second strands obtained during the asynchronous polymerization reactions. |
