Terpene synthases from <i>Santalum </i>

摘要

An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.

主权项

1. A method of making a terpene, comprising:
a) contacting an acyclic pyrophosphate terpene precursor with a terpene synthase encoded by a nucleic acid molecule to produce a terpene, wherein:
the nucleic acid molecule encodes a Santalum species terpene synthase that comprises:
(i) the sequence of amino acid resides set forth in SEQ ID NO:2, 4 or 6 or a catalytically active fragment thereof; or(ii) a sequence of amino acid residues that has at least 90% sequence identity to the sequence of amino acid residues set forth in SEQ ID NO: 2 or a catalytically active fragment thereof, wherein the encoded synthase catalyzes the production of a α-santalene, α-trans-bergamotene, epi-β-santalene and β-santalene concurrently; andif the method is performed in vivo in a cell, the nucleic acid is heterologous to the cell; and b) processing the terpene that is produced by contacting it with a P450 enzyme to produce a processed terpene.