Antibodies for the detection of integrin complexes in FFPE material

摘要

The invention relates to antibodies that are capable to bind the extracellular domain of integrin. Another object of the invention concerns the use of said antibodies for detecting integrins in archival formalin fixed paraffin embedded (FFPE) tissue. The invention also relates to methods for preparing monoclonal rabbit antibodies, wherein the immunogen is an insect expression culture-derived recombinant extracellular integrin domain, and another method for screening anti-integrin antibodies that discriminate between closest integrin homologues and that are especially suited for immunohistochemistry in FFPE material.

主权项

1. A monoclonal rabbit anti-integrin antibody or an integrin-binding fragment thereof, having specificity for an immunogen comprising an extracellular integrin domain said domain comprising an insect-derived glycosylation pattern, wherein the antibody or the integrin-binding fragment thereof is additionally capable of binding integrin with a eukaryotic glycosylation pattern other than derived from insects, wherein the antibody binds the immunogen integrin with an IC50≦5.6 ng/ml, wherein the IC50 of binding to the immunogen integrin is on the order of at least 10,000 times lower compared to the most closely related integrin.