Reaction device, reaction method and method of synthesizing cDNA

摘要

Provided is a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate. The projecting barrier has a cutoff portion and an inner space capable of holding a droplet and having a contact angle to pure water of from 90 to 150 degrees. A reaction method using the reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet by means of a magnet located on the opposite surface of the substrate to thereby conduct reactions and washings.

主权项

1. A reaction device comprising a plurality of U-shaped projecting barriers arranged in a line on a surface of a substrate,
wherein each U-shaped projecting barrier has two cutout portions, wherein openings formed by the two cutout portions have different sizes from each other, wherein each U-shaped projecting barrier substantially surrounds a space capable of holding a droplet, the space having a bottom defined by a first bottom surface, wherein the two cutout portions form a gap dividing each U-shaped projecting barrier, the gap having a bottom defined by a second bottom surface, wherein the first bottom surface, the second bottom surface, and a remainder of the surface of the substrate, other than the first bottom surface and the second bottom surface, are substantially level with each other, wherein at least the first bottom surface has pure water contact angle of 90 to 150 degrees; and wherein the reaction device is used in such a way that the surface having the plurality of U-shaped projecting barriers is downward.