摘要 |
PROBLEM TO BE SOLVED: To provide a protein with high enzyme activity which converts tuliposide B and analogs thereof into tulipalin B among the enzymes which converts tuliposides into tulipalins which is one of α-methylene-γ-butyrolactones and is useful as a monomer for heat-resistant transparent resin or a synthetic intermediate of a physiologically active substance, or a noxious insect repellent or an antimutagen agent, and to provide a polynucleotide encoding the same.SOLUTION: The protein related to the invention is the following (A) or (B): (A) a protein having a sequence shown in a specific sequence (TgTCEB1) or a specific sequence (TgTCEB2); or (B) a protein with high enzyme activity which has an amino acid sequence comprising 1-20 of amino acids substituted, deleted, inserted or added in the amino acid sequence shown in (TgTCEB1), or a specific sequence (TgTCEB2), and converts tuliposide B and analogs thereof into tulipalin B. |