METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS

摘要

Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

主权项

1. A method for detecting and/or classifying a colorectal carcinoma or pre-cancerous colorectal cell proliferative disorder in a human subject, comprising:
contacting genomic DNA from a biological sample obtained from a human subject, having a colorectal carcinoma or pre-cancerous colorectal cell proliferative disorder, with at least one agent that provides for determination of a CpG methylation status of the TMEFF2 gene; determining, based on the contacting, a CpG methylation status of the TMEFF2 gene; and detecting and/or classifying a colorectal carcinoma or precancerous colorectal cell proliferative disorder in the subject based on increased CpG methylation of the TMEFF2 gene, relative to that of a control sample or standard value.