Low-Volume Sequencing System and Method of Use

摘要

Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

主权项

1. A low-volume sequencing system, comprising:
a low-volume flowcell having at least one reaction chamber of a defined volume and at least one fluid transfer port formed therein; and an automated reagent delivery mechanism having a fluid dispenser with an internal compartment configured to retain a selected amount of reagent, the fluid dispenser having a distal portion configured to reversibly couple with the inlet port thereby placing the internal compartment into fluid communication with the at least one reaction chamber, and further configured to dispense to the reaction chamber a volume substantially equal to the defined volume of the reaction chamber.