MEASUREMENT OF NUCLEIC ACID VARIANTS USING HIGHLY-MULTIPLEXED ERROR-SUPPRESSED DEEP SEQUENCING

摘要

Methods and compositions are disclosed for measuring low-abundance DNA variants from a complex mixture of DNA molecules. Embodiments of the methods allow for extremely sensitive detection and can distinguish true variants from sequencer misreads and PCR misincorporations.

主权项

1. A method of measuring nucleic acid variants comprising
amplifying a low abundance template nucleic acid in a multiplexed polymerase chain reaction (PCR), wherein the PCR comprises gene specific primers comprising a) a barcode; and b) a molecular lineage tag (MLT), wherein the gene specific primers are specific for a mutation prone region.