METHOD FOR GENERATION OF CONDITIONALLY IMMORTALIZED HEMATOPOIETIC PROGENITOR CELL LINES WITH MULTIPLE LINEAGE POTENTIAL

摘要

The present invention is a method and kits for generating a homogenous population of hematopoietic progenitor cells capable of differentiating into a hematopoietic cell lineage. Whereas the combination of Homeobox-B8 protein and FMS-like tyrosine kinase 3 ligand generate cells with the potential to differentiate into different myeloid and lymphoid cell types, Homeobox-A7 protein and erythropoietin generate cells with the potential to differentiate into erythropoietic or thrombopoietic cell types.

主权项

1. A method for generating a homogenous population of hematopoietic progenitor cells capable of differentiating into hematopoietic cell lineages comprising expressing a hormone-regulated Homeobox (Hox) protein in a population of bone marrow cells, contacting the bone marrow cells with FMS-like tyrosine kinase 3 ligand (FLT3L) or erythropoietin, and culturing the cells in the presence of hormone thereby generating a homogenous population of hematopoietic progenitor cells capable of differentiating into different hematopoietic cell lineages.