METHOD FOR DETECTION OF MICROORGANISM AND KIT FOR DETECTION OF MICROORGANISM

摘要

Live cells of a microorganism in a test sample are detected by the following steps:
a) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison,b) the step of extracting DNA from the test sample, and amplifying a target region of the extracted DNA by PCR, andc) the step of analyzing an amplification product.

主权项

1. A method for detecting live cells by distinguishing the live cells from dead cells and also from injured cells of a microorganism in a test sample, which comprises the following steps:
a) treating the test sample with ethidium monoazide and irradiating the test sample with visible light; b) extracting DNA from the test sample and amplifying a target region of the extracted DNA by PCR; c) analyzing a resulting PCR amplification product; and d) distinguishing the live cells from dead cells and also from injured cells.