DEVICE AND METHOD FOR ANALYZING STREPTAVIDIN

摘要

The present invention provides a novel sensor for detecting streptavidin (SA). The nucleic acid sensor for analyzing SA of the present invention includes the following nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to SA. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (SA), a loop-forming sequence (L2), and a stem-forming sequence (SD) linked in this order. In this nucleic acid element, in the absence of SA, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (SA) and (SD), and in the presence of SA, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the SA, and the catalytic function of the catalyst nucleic acid molecule (D) is exerted.

主权项

1. A nucleic acid sensor for analyzing streptavidin, comprising the following nucleic acid element (I), (II), (II′), or (III) that comprises a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to streptavidin,
(I) a double-stranded nucleic acid element comprising a first strand and a second strand, the first strand (ss1) comprising the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order, the second strand (ss2) comprising a stem-forming sequence (SA), a loop-forming sequence (L2), and a stem-forming sequence (SD) linked in this order, wherein
a terminal region of the binding nucleic acid molecule (A) in the first strand (ss1) on the loop-forming sequence (L1) side is complementary to the stem-forming sequence (SA) in the second strand (ss2),a terminal region of the catalyst nucleic acid molecule (D) in the first strand (ss1) on the loop-forming sequence (L1) side is complementary to the stem-forming sequence (SD) in the second strand (ss2),the loop-forming sequence (L1) in the first strand (ss1) is non-complementary to the loop-forming sequence (L2) in the second strand (ss2), andin the absence of streptavidin, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (SA) and (SD), and in the presence of streptavidin, the stem formation in each of the stem-forming sequences (SA) and (SD) is released by a binding of the streptavidin with the binding nucleic acid molecule (A), and the catalytic function of the catalyst nucleic acid molecule (D) is exerted, (II) a single-stranded nucleic acid element comprising the binding nucleic acid molecule (A), a loop-forming sequence (L1), a stem-forming sequence (SD), the catalyst nucleic acid molecule (D), a loop-forming sequence (L2), and a stem-forming sequence (SA) linked in this order, wherein
a terminal region of the binding nucleic acid molecule (A) on the loop-forming sequence (L1) side is complementary to the stem-forming sequence (SA),a terminal region of the catalyst nucleic acid molecule (D) on the loop-forming sequence (L2) side is complementary to the stem-forming sequence (SD),the loop-forming sequence (L1) is non-complementary to the loop-forming sequence (L2), andin the absence of streptavidin, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (SA) and (SD), and in the presence of streptavidin, the stem formation in each of the stem-forming sequences (SA) and (SD) is released by a binding of the streptavidin with the binding nucleic acid molecule (A), and the catalytic function of the catalyst nucleic acid molecule (D) is exerted, (II′) a single-stranded nucleic acid element comprising the catalyst nucleic acid molecule (D), a loop-forming sequence (L2), a stem-forming sequence (SA), the binding nucleic acid molecule (A), a loop-forming sequence (L1), and a stem-forming sequence (SD) linked in this order, wherein
a terminal region of the catalyst nucleic acid molecule (D) on the loop-forming sequence (L2) side is complementary to the stem-forming sequence (SD),a terminal region of the binding nucleic acid molecule (A) on the loop-forming sequence (L1) side is complementary to the stem-forming sequence (SA),the loop-forming sequence (L1) is non-complementary to the loop-forming sequence (L2), andin the absence of streptavidin, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (SA) and (SD), and in the presence of streptavidin, the stem formation in each of the stem-forming sequences (SA) and (SD) is released by a binding of the streptavidin with the binding nucleic acid molecule (A), and the catalytic function of the catalyst nucleic acid molecule (D) is exerted, and (III) a single-stranded nucleic acid element comprising the catalyst nucleic acid molecule (D), an intervening sequence (I), and the binding nucleic acid molecule (A) linked in this order, wherein
the intervening sequence (I) is non-complementary to the catalyst nucleic acid molecule (D) and the binding nucleic acid molecule (A), andin the absence of streptavidin, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited, and in the presence of streptavidin the catalytic function of the catalyst nucleic acid molecule (D) is exerted.