Sample analysis systems, devices, and associated methods of operation

摘要

Embodiments of analysis systems, electrophoresis devices, and associated methods of analysis are described herein. In one embodiment, a method of analyzing a sample containing an electrolyte includes sequentially introducing a leading electrolyte, a sample electrolyte, and a trailing electrolyte into a extraction channel carried by a substrate. The extraction channel has a constriction in cross-sectional area. The method also includes applying an electrical field to separate components of the sample electrolyte into stacks and to concentrate the separated components by forcing the sample electrolyte to migrate through the constriction in the extraction channel. Thereafter, the applied electrical field is removed and the separated and concentrated components of the sample are detected in a detection channel carried by the substrate.

主权项

1. An electrophoresis device, comprising:
a first electrode having a first polarity; a second electrode having a second polarity opposite the first polarity; a substrate including
a sample reservoir configured to store a sample having a target component;an extraction channel in fluid communication to the sample reservoir, the extraction channel having a first section with a first cross-sectional area and a second section with a second cross-sectional area between a first end and a second end, the first end being electrically coupled to the first electrode and the second end being electrically coupled to the second electrode, wherein the first cross-sectional area is greater than the second cross-sectional area in a first dimension and in a second dimension generally orthogonal to the first dimension; anda detection channel in fluid communication with the second section of the extraction channel, the detection channel having a detection site configured to capture the target component in the extraction channel, wherein the detection site includes a cross-linked polymeric matrix carrying at least one of a marker, an anti-body, or an enzyme configured to react with the target component.