发明名称 |
METHOD OF PRODUCTION OF PEPTIDES |
摘要 |
FIELD: biotechnology.SUBSTANCE: method of production of peptides is proposed. Yeast autolysis is carried out. The cell membranes are separated by centrifugation. The autolysate is purified on the gel sulphocationite in the hydrogen form, containing 12-16% divinylbenzene. The resulting peptide aqueous solution is passed sequentially through the gel anion-exchange material to obtain the solution at pH 2.0-2.6, and then through the gel cation-exchange material with the divinylbenzene content of 1-2% or macroporous cation-exchange material.EFFECT: obtaining highly purified peptides that have biological activity.11 ex |
申请公布号 |
RU2544959(C1) |
申请公布日期 |
2015.03.20 |
申请号 |
RU20140113124 |
申请日期 |
2014.04.04 |
申请人 |
BUBNOV ALEKSANDR VLADIMIROVICH;OSTROVSKIJ DAVID ISAAKOVICH;RJAZANOV EVGENIJ MIKHAJLOVICH |
发明人 |
BUBNOV ALEKSANDR VLADIMIROVICH;OSTROVSKIJ DAVID ISAAKOVICH;RJAZANOV EVGENIJ MIKHAJLOVICH |
分类号 |
C07K1/18 |
主分类号 |
C07K1/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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