发明名称 |
APPLICATION OF OLIGO-DT MOLECULES TO AVOID GENERATION OF HIGH MOLECULAR PCR PRODUCTS INDUCED BY POLYA-CARRIER |
摘要 |
During RNA isolation carrier nucleic acids such as polyA-RNA are used in order to increase the yield of the isolated RNA. The carrier nucleic acids may lead to high molecular weight products which may interfere in subsequent steps, such as reverse transcription, PCR and gel electrophoresis. The present invention therefore refers to a method and a kit for reverse transcription and the use of a blocking nucleic acid molecule for blocking the carrier polyA-RNA. |
申请公布号 |
WO2015036434(A1) |
申请公布日期 |
2015.03.19 |
申请号 |
WO2014EP69294 |
申请日期 |
2014.09.10 |
申请人 |
F. HOFFMANN-LA ROCHE AG;ROCHE DIAGOSTICS GMBH |
发明人 |
BERGMANN, FRANK;FROEHNER, STEFANIE |
分类号 |
C12Q1/68;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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