摘要 |
<p>PROBLEM TO BE SOLVED: To provide a cartridge for nucleic acid amplification reaction that can easily cause nucleic acid amplification reaction.SOLUTION: A cartridge for nucleic acid amplification reaction comprises: a tube including in the inside a first plug comprising a first oil, a second plug comprising a cleaning solution, which causes phase separation when mixed with an oil and cleans a nucleic acid binding solid-phase carrier bound with nucleic acid, a third plug comprising a second oil, a fourth plug comprising an eluate, which causes phase separation when mixed with an oil and elutes the nucleic acid from the nucleic acid binding solid-phase carrier bound with the nucleic acid, and a fifth plug comprising a third oil, in this order; a container for nucleic acid amplification reaction in communication with the fifth plug side of the tube and including a fourth oil; and a plunger attached to an opening section on the first plug side of the tube and configured to push out a liquid from the fifth plug side of the tube to the container for nucleic acid amplification reaction. Each of the first to third oils has specific gravities different from the specific gravity of the fourth oil.</p> |