DNA SEQUENCING BY SYNTHESIS USING RAMAN AND INFRARED SPECTROSCOPY DETECTION

摘要

This invention provides nucleoside triphosphate analogues having the structure:;wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is an OH or an H, and wherein R′ is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl, and which has a Raman spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1 or a Fourier transform-infrared spectroscopy spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1, and also to methods of DNA sequencing and SNP detection.

主权项

1. A nucleoside triphosphate analogue having the structure: wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is OH or H, and wherein R′ is a hydrocarbyl, or a substituted hydrocarbyl other than an azidomethyl, which has a Raman spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1 or a Fourier transform-infrared spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1.