VARIANT HUMICOLA GRISEA CBH1.1

摘要

Disclosed are variants of Humicola grisea CeI7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

主权项

1. A polypeptide having cellobiohydrolase I activity, selected from the group consisting of
a) H. grisea CBH1.1 variant derived from CBS 225.63; b) H. grisea CBH1.1 variant having the sequence given in FIG. 3 (SEQ ID NO:3); c) Hypocrea jecorina CBH1 variant comprising at least one substitution selected from the group consisting of T55, S58, Q101, N250, P265 and L288 of the mature sequence; and d) Scytalidium thermophilium CBH1 (SEQ ID NO:11) derived from CBS 671.88.