METHODS FOR ISOLATING PROTEINS

摘要

The invention generally relates to methods for isolating proteins. In certain aspects, methods of the invention involve preparing a plurality of sample preparations, each preparation including one or more intact cells. A capture unit is introduced to a plurality of the preparations. The capture unit includes a member that transiently interacts with one or more proteins in the cells and a reactive functional group. The sample preparations are incubated, and a reaction is initiated at a different time in a plurality of the preparation. In this manner, a protein within the cell that specifically interacts with the member of the capture unit becomes bound to the capture unit via the reactive functional group to form protein/capture unit complexes. The cells are lysed and the protein/capture unit complexes are isolated.

主权项

1. A method for isolating a plurality of proteins, the method comprising:
preparing a plurality of sample preparations, each preparation comprising one or more intact cells; introducing a capture unit to a plurality of the preparations, the capture unit comprising a member that transiently interacts with one or more proteins in the cells and a reactive functional group; incubating the sample preparations; initiating a reaction at a different time in a plurality of the preparations such that a protein within the cell that specifically interacts with the member of the capture unit becomes bound to the capture unit via the reactive functional group to form protein/capture unit complexes; lysing the cells; and isolating the protein/capture unit complexes.