LASER SCANNING MICROSCOPE AND METHOD FOR CORRECTING IMAGING ERRORS PARTICULARLY IN HIGH-RESOLUTION SCANNING MICROSCOPY

摘要

A laser scanning microscope (SR-LSM) and a method for correcting imaging errors in a laser scanning microscope. The SR-LSM includes an illumination device for providing an illumination spot; a scanner for moving the illumination spot to consecutive scanning positions over a sample to be examined; an adaptive optics unit for controlling a wavefront of the illumination spot with a control device and a detector for determining a spatially resolved imaging spot emitted by the sample. An evaluation unit is provided for determining a point-spread function (PSF) of the imaging spot at each scanning position, whereby a wavefront correction signal determined from the point-spread function (PSF) of a scanning position is supplied to the control device of the adaptive optics unit or is used in digital post-processing of the microscope image (e.g. by means of deconvolution).

主权项

1. A laser scanning microscope comprising
an illumination device for providing an illumination spot; a scanner for moving the illumination spot over a sample to be examined to consecutive scanning positions; an adaptive optics unit for controlling a wavefront of the illumination spot with a control device; a detector for capturing a spatially-resolved imaging spot emitted by the sample; and an evaluation unit for determining a point spread function of the imaging spot in each scanning position, a wavefront correction signal determined from the point spread function of a previous or of the current seaming position being supplied to a control device.