METHODS AND COMPOSITIONS FOR SEPARATING RARE CELLS FROM FLUID SAMPLES

摘要

The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.

主权项

1. A method of enriching specific types of rare cells (e.g. fetal, cancer, stem, subpopulation of blood, etc.) from a body fluid sample (e.g. blood, ascites, urine, etc), comprising:
a) obtaining a sample; b) centrifuging said sample, optionally at a speed of between 10×g and 3000×g and time between 15 seconds and 30 minutes to obtain a sample wash pellet and a supernatant; c) resuspending said sample wash pellet to obtain a washed sample for rare cell enrichment; d) performing at least one debulking step on said washed sample for rare cell enrichment; e) and performing at least one separation on said washed sample for rare cell enrichment to obtain enriched rare cells.