| 发明名称 |
METHOD FOR INCREASING THE SECRETION OF RECOMBINANT PROTEINS |
| 摘要 |
The invention relates to a method for producing a recombinant protein in cells with a cell wall, comprising the step of increasing the secretion of the recombinant protein through the cell wall by expression of the protein in the cells in a culture medium containing a combination of a surface-active polymer and monovalent metal ions and with an osmolarity at least 0.32 osmol/L, said invention further relating to culture media and nutrient mixtures for the method. |
| 申请公布号 |
US2015079632(A1) |
申请公布日期 |
2015.03.19 |
| 申请号 |
US201314394991 |
申请日期 |
2013.04.17 |
| 申请人 |
GREENOVATION BIOTECH GMBH |
发明人 |
Jost Wolfgang;Knappenberger Mathias;Claussnitzer Doreen;Schaaf Andreas |
| 分类号 |
C12P21/00;C12N5/00 |
主分类号 |
C12P21/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. Method for producing a recombinant protein in cells having a cell wall, comprising the step of secreting the recombinant protein through the cell wall by means of expressing the protein present in the cells in a culture medium containing a combination of a surface-active polymer and monovalent metal ions and having an osmolarity of at least 0.32 osmol/L. |
| 地址 |
Freiburg DE |
