The present invention relates to a method for the determination of a nucleic acid sequence by physical manipulation. The method is based on the precise determination of the localization of the replicating fork on the template by measuring the physical distance between one end of the molecule and the fork. This allows the determination of the physical location of the site where a pause or a blockage of the replication occurs, and deducing therefrom information on the sequence of the nucleic acid.
申请公布号
EP2576822(B1)
申请公布日期
2015.03.18
申请号
EP20110722796
申请日期
2011.05.26
申请人
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);ECOLE NORMALE SUPÉRIEURE;UNIVERSITÉ PIERRE ET MARIE CURIE (PARIS 6)