Disposable cartridge for fluid analysis

摘要

A disposable blood analysis cartridge may include a sample collection reservoir, an absorbance measurement channel, and an optical light scattering measurement channel. One or more valves may be disposed between the sample collection reservoir and the absorbance measurement channel and/or the optical light scattering measurement channel. A negative pressure may be applied to the cartridge to pull sample from the sample collection reservoir through the one or more valves and into the absorbance measurement channel and/or the optical light scattering measurement channel. Once the sample is pulled into the absorbance measurement channel and/or the optical light scattering measurement channel, the one or more valves may be closed. With the one or more valves closed, and in some cases, a pusher fluid may be provided to push the fluid sample to other regions of the disposable fluid blood analysis cartridge.

主权项

1. A method of analyzing a blood sample in a cartridge, the method comprising:
receiving a blood sample via a blood sample introduction port of the cartridge, the blood sample being drawn into a sample collection reservoir by capillary action; applying a negative pressure to one or more vacuum ports of the cartridge, the negative pressure causing the blood sample to be drawn from the sample collection reservoir, through one or more open valves, and into a cuvette of an absorbance measurement channel and into at least part of an optical scattering measurement channel having a hydrodynamic focusing region; closing the one or more valves; receiving a reagent via a reagent introduction port, the reagent mixing with the blood sample in the optical scattering measurement channel upstream of the hydrodynamic focusing region; receiving a sheath fluid via a sheath fluid introduction port, the sheath fluid being injected at or near the hydrodynamic focusing region of the optical scattering measurement channel; performing an optical scatter measurement using a window that is adjacent the hydrodynamic focusing region of the optical scattering measurement channel; and performing an absorbance measurement using the cuvette of the absorbance measurement channel.