Tooth scaffolds

摘要

Provided is an acellular mammalian tooth-shaped scaffold including a compound that is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic. Also provided is a method of replacing a tooth in the mouth of a mammal, where the tooth is absent and a tooth socket is present in the mouth at the position of the absent tooth. The method includes implanting an acellular scaffold having the shape of the missing tooth into the tooth socket. Additionally, a method of making a tooth scaffold is provided. The method includes synthesizing an acellular scaffold in the shape of a mammalian tooth and adding at least one compound that is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic.

主权项

1. An acellular mammalian tooth-shaped scaffold comprising:
a matrix material; a composition comprising stromal cell-derived factor-1 (SDF-1) and a bone morphogenetic protein-7 (BMP-7); microchannels having a diameter of about 200 μm; and a nonporous cap; wherein
the composition is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic;the composition induces migration of progenitor cells when the scaffold is in contact with a dental tissue of a mammalian subject;the composition is distributed throughout the tooth-shaped scaffold; andthe scaffold does not comprise a living cell prior to implantation;BMP-7 is imbedded in the microchannels in a collagen gel at a concentration of about 100 ng/ml gel;SDF1 is imbedded in the microchannels in a collagen gel at a concentration of about 100 ng/ml gel; andthe scaffold is fabricated from the matrix material.