Minor groove binder (MGB)-oligonucleotide miRNA antagonists

摘要

Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.

主权项

1. A method of inhibiting miRNA activity in vitro or in vivo comprising introducing an inhibitor composition to a location in vitro or in vivo where miRNA activity exists;
wherein the inhibitor composition comprises: an oligonucleotide; and a minor groove binder (MGB), wherein the oligonucleotide comprises nucleotides, wherein all nucleotides of the oligonucleotide comprise ribofuranose having the following structure: wherein Rz is —OCH3, and wherein B is a normal base or a modified base, wherein the nucleotides are linked by natural phosphodiester linkages, and wherein the MGB is conjugated to the 5′ end of the oligonucleotide.