Biosensor, thin film electrode forming method, quantification apparatus, and quantification method

摘要

A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

主权项

1. A biosensor for quantifying a substrate in a sample liquid, the biosensor comprising:
an insulating support; an electrode pattern and an identification pattern formed of an electrically conductive material on a surface of the insulating support and having a thickness from 3 nm to 100 nm; a reagent layer covering a portion of the electrode pattern; a spacer layer covering a portion of the reagent layer; and a cover on the spacer layer;wherein:
the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer; a portion of the reagent layer is in the supply path, and the supply path is narrower than the reagent layer; the electrically conductive material comprises a noble metal; the electrode pattern defines at least a working electrode and a counter electrode; a portion of the working electrode and a portion of the counter electrode are in the supply path; and no portion of the identification pattern is in the supply path.