主权项 |
1. A recombinant microbial host cell comprising at least one modified DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion for each step below:
a) pyruvate to alpha acetolactate; b) alpha acetolactate to acetoin; c) acetoin to 2,3-butanediol; and d) 2,3-butanediol to 2-butanone;
and wherein said microbial host cell produces 2-butanol; andwherein the polypeptide that catalyzes a substrate to product conversion of 2,3-butanediol to 2-butanone is diol dehydratase or glycerol dehydratase, wherein the diol dehydratase or glycerol dehydratase comprises full length large, medium and small subunits that each give an E-value parameter of 0.01 or less when queried using a Profile Hidden Markov Model prepared using the large subunits of SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164; the medium subunits of SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; and the small subunits of SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; each query being carried out using the hmmsearch algorithm wherein the Z parameter is set to 1 billion. |