PROCESS FOR PRODUCING A CONCENTRATED SUGAR SOLUTION BY ENZYMATIC SACCHARIFICATION OF POLYSACCHARIDE ENRICHED BIOMASS

摘要

METHODS FOR OBTAINING CONCENTRATED SUGAR SOLUTION FROM POLYSACCHARIDE ENRICHED BIOMASS BY CONTACTING BIOMASS WITH WATER AND AT LEAST ONE NUCLEOPHILIC BASE TO PRODUCE A POLYSACEHARIDE ENRICHED BIOMASS COMPRISING A SOLID FRACTION AND A LIQUID FRACTION AND THEN CONTACTING THE SOLID FRACTION WITH SACCHARIFICATION ENZYME CONSORTIUM TO PRODUCE A SACCHARIFICATION PRODUCT COMPRISING AT LEAST ABOUT 7 PERCENT BY WEIGHT SUGARS IN 24 HOURS. THE METHODS INCLUDE OPTIONALLY ADDING AT LEAST ONE ADDITIVE SELECTED FROM THE GROUP CONSISTING OF POLYETHYLENE GLYCOLS, FATTY ACID ESTERS, FATTY ACID ETHOXYLATES, NONIONIC SURFACTANTS DERIVED FROM POLYETHOXYLATED SORBITAN AND A FATTY ACID, SODIUM LAURIMINODIPROPIONATE, SODIUM COCOAMPHOACETATE, SODIUM TRIDECYL ETHER SULFATE AND A COMBINATION OF THESE, SUCH THAT ENZYME LOADING OF THE SACCHARIFICATION ENZYME CONSORTIUM CAN BE REDUCED.