Microscope

摘要

A microscope including an imaging objective for imaging a sample on a detector and means for illuminating the sample with a light sheet in the focus plane of the imaging objective. The illumination means includes an illumination source which emits coherent light, and Bessel optics which generate at least two plane waves from the light beam and give propagation directions for the plane waves. The propagation direction of each of the plane waves encloses an acute angle with the focus plane in each instance, the magnitude of the acute angle being identical for each of the plane waves, so that the plane waves undergo constructive interference in the focus plane so that a light sheet is generated. Similarly, the illumination means can also include an optical element by which a rotationally symmetric Bessel beam is generated from the light beam for dynamic generation of a light sheet.

主权项

1. A microscope comprising:
an imaging objective for imaging a sample on a detector; and means for illuminating the sample with a light sheet in the focus plane of the imaging objective; wherein the illumination means comprise:
an illumination source which emits coherent light in the form of a light beam; andBessel optics which generate at least two plane waves from the light beam and give propagation directions for the at least two plane waves, respectively; and wherein the propagation direction of each of the plane waves encloses an acute angle with the focus plane in each instance, the magnitude of the acute angle being identical for each of the plane waves, so that the at least two plane waves undergo constructive interference in the focus plane so that a light sheet is generated.