Methods For Altering The Genome Of A Monocot Plant Cell

摘要

Methods and compositions for altering the genome of a monocot plant cell, and a monocot plant are disclosed. The methods and compositions use a double-strand break inducing agent to alter a monocot plant or plant cell genomic sequence comprising a recognition sequence for the double-strand break inducing agent.

主权项

1. A method to alter a monocot plant cell genome comprising:
(a) contacting at least one monocot plant cell comprising a recognition sequence in its genome with (i) a phenotypic marker, (ii) a DNA fragment, and (ii) a double-strand break inducing agent capable of inducing a double-strand break at the recognition sequence; (b) selecting cells comprising the phenotypic marker; (c) identifying cells from step (b) having an alteration in their genome at the recognition sequence wherein the alteration is selected from the group consisting of (i) replacement of at least one nucleotide, (ii) a deletion of at least one nucleotide, (iii) an insertion of at least one nucleotide, and (iv) any combination of (i)-(iii), wherein identifying comprises any method that does not use a phenotypic marker; and, (d) recovering a fertile monocot plant having the alteration in its genome.