NEW ANTIGEN FOR DETECTING REJECTION RESPONSE INCLUDING MINIATURE PIG PANCREAS PROTEIN ACCORDING TO DEVELOPMENTAL STAGES AND THE KIT FOR DETECTING REJECTION RESPONSE INCLUDIGN THE SAME

摘要

The present invention relates to a protein antigen capable of detecting the xenotransplantation rejection response between pig and human and a kit for detecting the xenotransplantation rejection response comprising the same, and more specifically, to an antigen for detecting the xenotransplantation rejection response comprising a protein antigen exhibiting the difference in the translation procedure according to the pig development stage and a kit for detecting the xenotransplantation rejection response. In the present invention, we reported the regulation of protein according to the pancreas development stage using 2-DE and MALDI-TOF (4-day newborn baby, 19-day pigling, and 14-month adult miniature pig). It was verified from images of three different stages that a total of 13 spots were differently regulated and 9 spots were MALDI-TOF MS. The data shown in the present invention provides an important direction in association with the development stage of the miniature pig pancreas, and thus will help the subsequent proteome analysis of pancreas and promote the understanding of obstacles to the xenotransplantation rejection response. The novel antigen which is differently regulated according to the development stage can be favorably used to detect the xenotransplantation rejection response.