MEDIUM FOR THE PROTEIN-FREE AND SERUM-FREE CULTIVATION OF CELLS

摘要

A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

主权项

1. A method of adapting recombinant cells to serum-free and animal protein-free medium, the method comprising:
a. culturing recombinant cells in serum-containing medium, b. transferring said cells into a serum-free and animal protein-free medium, wherein the medium comprises soy hydrolysate wherein at least 40% of said soy hydrolysate has a molecular weight of ≦500 Daltons, and c. culturing said cells in said serum-free and animal protein-free medium.