| 摘要 |
A method for identifying a nucleic acid segment which interacts with a target nucleic acid segment, said method comprising the steps of: obtaining a nucleic acid composition comprising the target nucleic acid segment, crosslinking the nucleic acid composition, fragmenting the crosslinked nucleic acid composition, ligating the fragmented nucleic acid segments to produce ligated fragments, adding an isolating nucleic acid molecule that binds to the target nucleic acid segment, wherein said isolating nucleic acid molecule is labelled with a first half of a binding pair, isolating ligated fragments which contain the target nucleic acid segment bound to the isolating nucleic acid molecule by using the second half of the binding pair; and sequencing these isolated ligated fragments to identify the nucleic acid segment which interacts with the target nucleic acid segment. The method can identify interacting nucleic acid segments that are indicative of a particular disease state. |
